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Cytokeratin 5/8(C-50), CF640R conjugate, 0.1mg/mL

Cytokeratin 5/8(C-50), CF640R conjugate, 0.1mg/mL

Item no.	B-BNC400948-100
Manufacturer	Biotium
Amount	100 uL
Quantity options	100 uL 500 uL
Category	Antibodies Cell Biology Cytoskeleton Primary Antibodies Intermediate filaments
Type	Antibody Primary
Format	Liquid
Applications	IHC
Clone	C-50
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Sheep (Ovine, Ovis aries), Hamster, Ferret, Dog (Canine, Canis lupus familiaris), Pig (Porcine, Sus scrofa domesticus), Cow (Bos taurus)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	CF640R
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CK5; DDD; EBS2; Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; K5; Keratin complex 2, basic, Gene 5; Keratin Type II Cytoskeletal 5; Keratin-5; KRT5; KRT5A; Type II Cytoskeletal 5; Type-II Keratin Kb5. CK8; CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8
Shipping Condition	Room temperature
Available
Manufacturer - Type	Primary
Manufacturer - Applications	IHC, FFPE (verified)
Manufacturer - Category	Antibodies
Manufacturer - Targets	Cytokeratin 5/8
Manufacturer - Conjugate / Tag	CF640R
Manufacturer - Host	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature	Room temperature
Storage Conditions	Store at 2 to 8°C\|Protect fluorescent conjugates from light
Molecular Weight	58 kDa (CK5); 52.5 kDa (CK8)
Manufacturer - Research Area	Cancer\|Cytoskeleton
Description	This antibody reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin	Animal
Ingredient of biological origin	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label	Protect from light
Undated stability guarantee in PI	2 years
Stability at RT during shipping (protected from light)	Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity	41116161
UNSPSC Commodity Title	Primary and secondary antibodies for multiple methodology immunostaining detection application
Classified or regulated chemicals	0.05% sodium azide (CAS 26628-22-8)
Concentration	0.1 mg/mL
Storage buffer	PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label	Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)	Cytoskeletal preparation from HeLa cells
Clonality	Monoclonal
Regulatory Status	For research use only (RUO)
Human Gene Symbol	KRT5
Unigene	433845 (CK5); 533782 & 708445 (CK8)
Positive Control	HepG2 or A431 cells. Skin or Squamous cell carcinoma
Antibody target cellular localization	Cytoskeleton
Cell tissue expression	Epithelial cells
Tumor expression	Breast cancer\|Colorectal cancer\|Lung cancer\|Ovarian cancer\|Thyroid cancer
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody\|Immunohistology formalin-fixed 0.5-1.0 ug/mL\|For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/mL PBS, 15 min at RT\|Flow Cytometry 0.5-1 ug/million cells/0.1 mL\|Immunofluorescence 0.5-1.0 ug/mL\|Western blotting 0.5-1.0 ug/mL\|Does not react with chicken , or Xenopus\|Optimal dilution for a specific application should be determined by user
Manufacturer - Synonym	CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8
Manufacturer - Antibody Reactivity	Cytokeratin 5/8

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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B-BNC400948-100-datasheet.pdf

B-BNC400948-100-safety-datasheet.pdf