Comparison

Human Nuclear Antigen(235-1), CF543 conjugate, 0.1mg/mL

Item no. B-BNC430099-500
Manufacturer Biotium
Amount 500 uL
Quantity options 100 uL 500 uL
Category
Type Antibody Primary
Format Liquid
Applications FC, IF, IHC
Clone 235-1
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Conjugate/Tag CF543
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HNA; HuNu
Shipping Condition Room temperature
Available
Specificity Human, Non-human primates
Manufacturer - Type
Primary
Manufacturer - Applications
Flow, intracellular (verified)|IF (verified)|IHC, frozen (verified)
Manufacturer - Category
Antibodies
Manufacturer - Targets
Human Nuclear Antigen
Manufacturer - Conjugate / Tag
CF543
Manufacturer - Host
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature
Room temperature
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Molecular Weight
70 kDa & 80 kDa
Manufacturer - Research Area
Organelle markers
Description
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Nuclei of human myeloid leukemia biopsy cells
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Positive Control
All human cells. Tonsil
Antibody target cellular localization
Nucleus
Antibody application notes
Immunohistology Frozen 0.5-1.0 ug/mL|Immunocytochemistry acetone-fixed cells 0.25-0.5 ug/mL|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunoprecipitation 0.5-1 ug/500 ug protein lysate|Does not react with mouse, rat or chicken; others not known|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
Human Nuclear Antigen

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 uL
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?