Comparison

IgM Immunoglobulin(IM260), CF647 conjugate, 0.1mg/mL

Item no. B-BNC470260-100
Manufacturer Biotium
Amount 100 uL
Quantity options 100 uL 500 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IHC
Clone IM260
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Conjugate/Tag CF647
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region
Shipping Condition Room temperature
Available
Manufacturer - Type
Anti-Human Immunoglobulin|Primary
Manufacturer - Applications
IHC, FFPE (verified)|WB (verified)
Manufacturer - Category
Antibodies
Manufacturer - Targets
Human IgM
Manufacturer - Conjugate / Tag
CF647
Manufacturer - Host
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature
Room temperature
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Molecular Weight
50-75 kDa
Manufacturer - Research Area
Cancer|Immunology|Inflammation
Description
Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Recombinant heavy chain of human IgM
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
IGHM
Unigene
510635
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Antibody target cellular localization
Plasma membrane|Secreted (extracellular)
Cell tissue expression
B-cells
Tumor expression
Leukemia/lymphoma
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
Human IgM

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 uL
Available: In stock
available

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