Napsin A (Lung Adenocarcinoma Marker)(NAPSA/1238 + NAPSA/1239), CF647 conjugate, 0.1mg/mL

Primary

Antibodies

CF647

Room temperature

37 kDa

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

2 years

41116161

0.05% sodium azide (CAS 26628-22-8)

PBS, 0.1% BSA, 0.05% azide

Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)

For research use only (RUO)

512843

Secreted (extracellular)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistochemistry (formalin-fixed): 1-2 ug/mL for 30 minutes at RT|Western blot: 1-2 ug/mL for 60 minutes at RT|Flow cytometry: 0.5-1 ug/million cells|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user