Comparison

bcl-x(BX006), CF488A conjugate, 0.1mg/mL

Item no. B-BNC880006-500
Manufacturer Biotium
Amount 500 uL
Quantity options 100 uL 500 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IHC
Clone BX006
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Pig (Porcine, Sus scrofa domesticus)
Host Mouse
Isotype IgG2a
Conjugate/Tag CF488A
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Bcl-x; Bcl-2-like protein 1; BCL2L1; Apoptosis regulator Bcl-X
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary
Manufacturer - Applications
IHC, FFPE (verified)|WB (verified)
Manufacturer - Category
Antibodies
Manufacturer - Targets
Bcl-x
Manufacturer - Conjugate / Tag
CF488A
Manufacturer - Host
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature
Room temperature
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Molecular Weight
27 kDa
Manufacturer - Research Area
Apoptosis|Cancer
Description
Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
BCL2L1
Unigene
516966
Positive Control
Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.
Antibody target cellular localization
Cytoplasmic|Endoplasmic reticulum|Mitochondria
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
Bcl-x

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 uL
Available: In stock
available

Delivery expected until 8/28/2025 

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