Blood Group H Type 2 (CD173)(19-OLE), 0.2mg/mL

Primary

Antibodies

Purified, with BSA

Room temperature

Multiple

This antibody is applicable for staining ABO blood group O. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. This antibody recognizes the blood group H type 2 antigens, trisaccharide Fuc-a-1-2Gal1-4GlcNAc-a-1. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodology
immunostaining detection application

0.2 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

ABO

KG1 cells or human colorectal carcinoma.

Immunohistology (formalin)|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user