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Actinin-alpha1/2/3/4 (W48) polyclonal antibody

Actinin-alpha1/2/3/4 (W48) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Neuroscience Neural Lineage Markers Protein Aggregation, Clearance & Misfolding Antibodies Immunohistochemistry Western Blot Cardiovascular Research Hypertension Primary Antibodies Cytoskeleton Markers
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB, IHC
Amount	100ul
Host	Rabbit
Item no.	BS2206-100
Targets	ACTN1;;ACTN4;;ADRA1D;;IGBP1
eClass 6.1	32160702
eClass 9.0	32160702
Available
Swiss-Prot	P12814/P35609/Q08043/O43707
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200
Background	The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and alpha-actinins. There are four tissue-specific alpha-actinins, namely alpha-actinin-1, alpha-actinin-2, alpha-actinin-3 and alpha-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each alpha-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding alpha-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	Actinin-alpha1/2/3/4 (W48) pAb detects endogenous levels of total alpha-actinin protein.
WB	Western blot (WB) analysis of Actinin-alpha1/2/3/4 (W48) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse muscle tissue lysate Lane3:Rat muscle tissue lysate
BiowMW	ca. 103 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	pan-alpha-Actinin, alpha-Actinin-pan, Actinin-alpha, Actinin alpha, alpha Actinin, alpha-Actinin, Alpha-actinin-4, F-actin cross-linking protein, Non-muscle alpha-actinin 4, ACTN4, alpha-Actinin4, Actinin alpha4, Actininalpha4, alphaActinin-4, Actinin-alpha4, alpha-actinin-4, Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, Non-muscle alpha-actinin-1, ACTN1, alpha-Actinin1, Actinin alpha1, Actininalpha1, alphaActinin-1, Actinin-alpha1, alpha-actinin-1, Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, ACTN2, alpha-Actinin2, Actinin alpha2, Actininalpha2, alphaActinin-2, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, ACTN3, alpha-Actinin3, Actinin alpha3, Actininalpha3, alphaActinin-3, Actinin-alpha3, alpha-actinin-2, alpha-actinin-3
Immunogen	Synthetic peptide, corresponding to amino acids 30-60 of Human Actinin-alpha1.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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