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Histone H3 (Phospho-S10) polyclonal antibody

Histone H3 (Phospho-S10) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Antibodies Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Primary
Specific against	Human, Mouse, Rat
Applications	WB, IF, IP, IHC, ICC
Amount	50ul
Host	Rabbit
Item no.	BS94004-50
Targets	H3-3A
eClass 6.1	32160702
eClass 9.0	32160702
Available
Swiss-Prot	P68431(Human) P84243(Human) Q16695(Human) Q6NXT2(Human) Q71DI3(Human) P84244(Mouse) P84245(Rat)
Application_all	WB:1:1, 000-1:5, 000 ICC:1:50-1:200 IHC:1:50-1:200
Background	In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3. The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro.
Product	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification And Purity	ProA affinity purified
Storage And Stability	Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Specificity	Histone H3 (Phospho-S10) polyclonal antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.
BiowMW	15 kDa
Note	For research use only, not for use in diagnostic procedure.
Immunogen	Synthetic phospho-peptide corresponding to residues surrounding Ser10 of human Histone H3.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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