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eIF4E (Phospho-S209) polyclonal antibody

eIF4E (Phospho-S209) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Antibodies Immunohistochemistry Immunoprecipitation Western Blot Immunocytochemistry Primary Antibodies
Type	Antibody Primary
Specific against	Human, Mouse, Rat
Applications	WB, IP, IHC, ICC
Amount	50ul
Host	Rabbit
Item no.	BS94012-50
Targets	EIF4E
eClass 6.1	32160702
eClass 9.0	32160702
Available
Swiss-Prot	P06730(Human) P63073(Mouse) P63074(Rat)
Application_all	WB:1:1, 000-1:5, 000 ICC:1:50-1:200 IHC:1:50-1:200
Background	The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.
Product	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification And Purity	ProA affinity purified
Storage And Stability	Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Specificity	eIF4E (Phospho-S209) polyclonal antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.
BiowMW	25 kDa
Note	For research use only, not for use in diagnostic procedure.
Immunogen	Synthetic phospho-peptide corresponding to residues surrounding Ser209 of human eIF4E.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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