Histone H2A.X (Ab-139) Antibody

Polyclonal Antibody

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Peptide sequence around aa.137ca.141 (Q-A-S-Q-E) derived from Human Histone H2A.X.

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript, available in PMC 2006 March 6

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugateds. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Epigenetics and Nuclear Signaling

Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.