Phospho-Tuberin (Thr1462) Polyclonal Antibody

Store at -20C. Avoid freeze / thaw cycles.

200kDa

In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.

PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000