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Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]

Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]

Item no.	E-AB-F1112A-25
Manufacturer	Elabscience
Amount	25 ug
Quantity options	100 ug 1 mg 25 ug 50 ug 5 mg
Category	Antibodies Primary Antibodies
Type	Antibody Monoclonal
Applications	FC
Clone	RA3.3A 1/6.1
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgM Kappa
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	B220,
Shipping Condition	Cool pack
Available
Manufacturer - Category	Flow Cytometry Antibodies
Manufacturer - Isotype	Rat IgM, κ
Shipping Temperature	Ice bag
Storage Conditions	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
Background	CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.
Concentration	≥1 mg/mL
Buffer	Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300.
Abbreviation	CD45R/B220
Clonality	Monoclonal
Usage	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10^6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.