PARP1 Antibody

WB 1:500-1:1000
IHC 1:50-1:100

Store at -20C/1 year

Recombinant protein of human PARP1

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2, 3) and is one of the main cleavage targets of caspase-3 in vivo (4, 5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2, 4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

EN0043447