Item no. |
FNab00869-100ug |
Manufacturer |
FineTest
|
Amount |
100ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
liquid |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus) |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, beta actin |
Available |
|
Purification |
Immunogen affinity purified |
Purity |
>=95% as determined by SDS-PAGE |
Clonality |
polyclonal |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20C for 12 months(Avoid repeated freeze / thaw cycles.) |
Background |
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. |
Immunogen |
actin, beta |
Observed MW |
42 kDa |
Uniprot ID |
P60709 |
Tested Application |
ELISA, IF, WB, IHC |
Recommended dilution |
WB: 1:500-1:2000; IHC: 1:20-1:500; IF: 1:10-1:100 |
Research Area |
Cell Division and Proliferation, Cardiovascular, Metabolism |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.