Manufacturer |
IchorBio
|
Category |
|
Type |
Antibody |
Specific against |
Human |
Clone |
CD28.2 |
Isotype |
IgG1 Kappa |
Applications |
FC, IHC |
Amount |
0.5mg |
Host |
Mouse |
Item no. |
ICH1013-0.5MG |
Targets |
CD28 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Aggregation |
Aggregation level <= 5% |
Antigen Distribution |
Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-gamma |
Concentration |
1.0 mg/ml |
Endotoxin |
<0.1 EU/mg as determined by the LAL method |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Product Type |
Low Endotoxin, anti-human |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Storage |
anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2) is stable for at least one week when stored sterile at 2-8C. For long term storage aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.