« Back
Home /
Anti-Mouse CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11)

Anti-Mouse CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11)

Manufacturer	IchorBio
Category	In Vivo Antibodies In Vivo Antibodies Flow Cytometry Immunohistochemistry Western Blot Blocking
Type	Antibody
Specific against	Mouse
Clone	145-2C11
Isotype	IgG
Applications	WB, FC, IP, IHC, Blocking, IV
Amount	25mg
Host	Hamster - Armenian
Item no.	ICH1041UL-25MG
Targets	CD3e
eClass 6.1	32160702
eClass 9.0	32160702
Available
Manufacturers Product Category	Ultra-Low Endotoxin, anti-mouse
Isotype Information	Armenian Hamster IgG
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution	The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.
Background	CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Immunogen	H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Concentration	1.0 - 5.0 mg/ml
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Aggregation	Aggregation level ≤ 1%
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: - Mycoplasma spp - Mycoplasma pulmonis - Pneumonia virus of mice - Kilham's rat virus - Toolan's H1 virus - Hamster parvovirus - Lymphocytic choriomeningitis - Minute virus of mice - Theiler's murine encephalomyelitis virus - Sendai virus - Reovirus 3 - Hantaan virus
Storage	anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Application Notes	Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells - Each investigator should determine their own optimal working dilution for specific applications.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 25mg

available

Delivery expected until 6/6/2024