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Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

Item no.	ICH1046-1MG
Manufacturer	IchorBio
Amount	1 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	Oncology Antibodies In Vivo Antibodies By Cell Type Hematology Leukemia Western Blot
Type	Antibody
Applications	WB, FC, IP, IHC, ICC, FA
Clone	53-5.8
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG1
Purity	>95% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3
Shipping Condition	Cool pack
Available
Specificity	Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1
Manufacturer - Type	Low Endotoxin, anti-mouse
Manufacturer - Applications	Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation
Manufacturer - Category	Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG1
Manufacturer - Targets	CD8 beta
Shipping Temperature	Blue ice
Storage Conditions	anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 5%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	Majority of thymocytes, cytotoxic T cell subsets
Application Notes	Each investigator should determine their own optimal working dilution for specific applications.
Background	CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.
Concentration	1.0 - 5.0 mg/ml
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Mouse thymus or spleen
Related Antibodies	Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin [53-5.8] (ICH1046UL)
Isotype Control	Rat IgG1 Isotype Control for In Vivo - Low Endotoxin [GL113] (ICH2246)
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 mg

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Delivery expected until 9/4/2025

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