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Anti-Mouse IL-4 (11B11) In Vivo Antibody - Ultra-Low Endotoxin

Anti-Mouse IL-4 (11B11) In Vivo Antibody - Ultra-Low Endotoxin

Item no.	ICH1087UL-100MG
Manufacturer	IchorBio
Amount	100 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies
Type	Antibody
Applications	FC, IP, IHC, ELISA, FA, NT
Clone	11B11
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG1 Kappa
Purity	>98% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Interleukin-4, Il-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1
Shipping Condition	Cool pack
Available
Specificity	Anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4
Manufacturer - Applications	ELISA Capture, ELISPOT, Flow Cytometry, Neutralization, Immunoprecipitation, Functional Assays, CyTOF, IHC
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG1 Kappa
Manufacturer - Targets	IL-4
Shipping Temperature	Blue ice
Storage Conditions	anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) is stable when stored at 2-8°C for 1 week. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Application Notes	ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml. ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 µg/ml is recommended. Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Background	Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.
Concentration	1.0 - 5.0 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-IL-4 In Vivo Antibody - Ultra-Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Purified Recombinant Mouse IL-4 (>98%)
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus
Antibodies against the same target (Cat No.)	ICH1102-0.5MG, ICH1030-0.5MG
Isotype Control	Rat IgG1 Isotype Control for In Vivo - Low Endotoxin [GL113] (ICH2246)
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 mg

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Delivery expected until 10/2/2025

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