Comparison

Bulk Anti-Human CD2 (LO-CD2a) In Vivo Antibody European Partner

Item no. ICH1193-25mg
Manufacturer IchorBio
Amount 25 mg
Quantity options 100 mg 25 mg 50 mg 5 mg
Category
Type Antibody Monoclonal
Applications FC, ELISA, FA
Clone LO-CD2a
Specific against Human (Homo sapiens)
Host Rat
Isotype IgG2b Kappa
Purity >95% by SDS-PAGE
Formula Sterile, preservative-free, solution in PBS. BSA and Azide free.
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias BTI-322, CD2, CD2 molecule, T11, SRBC, LFA-2, T-cell surface antigen CD2, LFA-3 receptor, rosette receptor, erythrocyte receptor, lymphocyte-function antigen-2, T-cell surface antigen T11/Leu-5, CD2 antigen (p50), sheep red blood cell receptor
Shipping Condition Cool pack
Available
Specificity Anti-CD2 antibody (clone LO-CD2a) activity is directed against human CD2.
Manufacturer - Applications
ELISA, Functional Assays, Flow Cytometry
Manufacturer - Category
Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2b Kappa
Manufacturer - Targets
CD2
Shipping Temperature
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 5%
Formulation
Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution
CD2 is expressed on all mature human T cells, most thymocytes, NK cells and a small proportion of bone marrow cells.
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Background
LO-CD2a strongly inhibits mixed lymphocyte culture (MLC) 3 and induces T cell apoptosis. Additionally, LO-CD2a does not induce proliferation of resting T cells, and inhibits T cell proliferation induced by OKT3 antibody, induces very low levels of cytokine release, and also inhibits cytokine production induced by OKT3, LO-CD2a strongly inhibits proliferation of peripheral blood mononuclear cells stimulated with soluble OKT3, and induces human T cell hyporesponsiveness. Adding LO-CD2a to a primary MLC inhibits the T cell proliferation response and has a subsequent inhibitory effect on the secondary immune response, thereby blocking primary and memory alloantigen proliferative responses. The human T cell hyporesponsiveness in the secondary MLC is restricted to alloantigens and occurs both in the absence of antibody and after cell surface re-expression of CD2
Concentration
≥ 2.0 mg/ml
Endotoxin
<1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-CD2 antibody (clone LO-CD2a) activity is directed against human CD2.
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
LO-CD2a was developed as an immunosuppressive agent for use in organ allotransplantation. LO-CD2a was produced by immunizing a LOU/C rat with purified human T lymphocytes and fusing the splenocytes with the non-secreting rat fusion cell line IR983F, resulting in a rat IgG2b mAb of the IgK-1a allotype
Isotype Control
Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
Buffer
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 mg
Available: In stock
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