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Anti-Mouse CD49d (PS/2) In Vivo Antibody - Ultra-Low Endotoxin

Anti-Mouse CD49d (PS/2) In Vivo Antibody - Ultra-Low Endotoxin

Item no.	ICH1200UL-5mg
Manufacturer	IchorBio
Amount	5 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IP, IHC, FA
Clone	PS/2
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2b Kappa
Purity	>98% by SDS-PAGE and HPLC
Formula	Sterile, preservative-free, solution in PBS. BSA and Azide free.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	VLA-4α, ITGA4, Integrin α4
Shipping Condition	Cool pack
Available
Specificity	PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.
Manufacturer - Applications	Functional Assays, Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG2b Kappa
Manufacturer - Targets	CD49d
Shipping Temperature	Blue ice
Storage Conditions	This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution	CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Application Notes	Each investigator should determine their own optimal working dilution for specific applications.
Background	Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture.
Concentration	≥ 2.0 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0.
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus
Isotype Control	Rat IgG2b In Vivo Isotype Control – Low Endotoxin (1-2) [ICH2243]
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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