Comparison

Anti-Mouse Adiponectin - 25 µg

Item no. LEIN-A355-25ug
Manufacturer Leinco Technologies
Amount 25 ug
Quantity options 100 ug 25 ug
Category
Type Antibody Primary
Applications WB, ICC
Specific against Mouse (Murine, Mus musculus)
Host Caprine
NCBI 11450
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Shipping Condition Room temperature
Available
Manufacturer - Applications
Quality Tested by Leinco Western Blotting: To detect Mouse Adiponectin this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Adiponectin is 1.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is A411.
Manufacturer - Category
Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets
ACRP30
Country of Origin
USA
Shipping Temperature
Next Day Ambient
Storage Conditions
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease. Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors. Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes. The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities. Adiponectin peptide is applicable as a control peptide for blocking antibody binding in Western blotting.
Background
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease. Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors. Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes. The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities.
Adiponectin peptide is applicable as a control peptide for blocking antibody binding in Western blotting.
Other Applications Reported In Literature
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
PubMed
ACRP30
Manufacturer - Specificity
Goat Anti-Mouse Adiponectin recognizes Mouse Adiponectin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID
AB_2828105
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Immunogen
NS0-derived mouse Adiponectin
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords
Polyclonal Antibody, Adiponectin, ADIPOQ, ACDC, ACRP30, APM-1, APM1, GBP28, Mouse Adiponectin, Anti-Mouse Adiponectin, ACDC, Acrp30, ACRP30ADPN, adipocyte, C1Q and collagen domain containing, Adiponectin, adiponectin, C1Q and collagen domain containing, AdipoQ, ADIPQTL1, ApM1, apM-1, APM1APM-1, C1q and collagen domain-containing protein, GBP28, GBP28apM1, Gelatin-binding protein

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 ug
Available: In stock
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