Anti-Human Amphiregulin (Clone 31221) - 100 µg

ELISA Cap, N, WB

Amphiregulin

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Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-α, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor.

Neutralization: This antibody is useful for neutralization of Human AR bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human AR (at 50 ng/ml) is 5-15 µg/ml.

Mouse Anti-Human Amphiregulin (AR) (Clone 31221) recognizes an epitope on Human AR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 6.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Purified Recombinant Human AR (> 98%)

Monoclonal Antibody, Amphiregulin, SDGF, 31221, Human, Anti-Human Amphiregulin, AR, AREG, AREGB, Colorectum cell-derived growth factor, CRDGF, MGC13647, schwannoma-derived growth factor