Anti-Human PD-L1 (CD274) (Clone 130021) - 25 µg

CyTOF®, FC, IHC

PD-L1

Next Day Ambient

PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a coinhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.

Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Clone 130021 recognizes an epitope on human B7-H1. In direct ELISAs, this antibody shows no cross-reactivity with rhB7-1, rhB7-2, rhB7-H2, rhB7-H3, rmB7-H1 or rmPD-L2.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 6.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Purified Recombinant Human CD274/PD-L1 (Accession # Q9NZQ7)

B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274 antigenMGC142294, CD274 molecule, CD274, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H, B7-H1, B7H1, human CD274, CD274 antigen, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, human Programmed cell death 1 ligand 1, human programmed death ligand 1, A530045L16Rik, B7h1, Cd274, Pdcd1l1, Pdcd1lg1, human Pdl1