Anti-Human CD27 - 100 µg

CyTOF®, FC, IHC, N, WB

CD27

Next Day Ambient

CD27 is a tumor necrosis factor receptor. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It exists as both a disulfide-linked dimer on the cell surface and as a soluble protein found in serum. It binds to ligand CD70 which is expressed on thymic stromal cells and a small subset of activated T cells and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Neutralization: This antibody is useful for neutralization of Human CD27 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CD27 (at 3 ng/ml) is 2.25-9 µg/ml.
Immunohistochemistry: Suitable for use at concentration of 3-15 µg/mL.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Goat Anti-Human CD27 recognizes Human CD27. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Purified Recombinant Human CD27 (> 98%)

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