Anti-Human CD30 (Clone 81316) - 500 µg

Quality Tested by Leinco Western Blotting: To detect Human CD30 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CD30 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1357.

CD30

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CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily and tumor marker. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. It is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. CD30 is associated with anaplastic large cell lymphoma. It is expressed in embryonal carcinoma but not in seminoma and is thus is a useful marker in distinguishing between these germ cell tumors.

Immunohistochemistry: Suitable for use at concentration of5-25 µg/mL.

Mouse Anti-Human CD30 (Clone 81316) recognizes an epitope on Human CD30. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rh4-1BB, rhCD27, rmCD30, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhHVEM, rhLTβ R, rhNGF R, rhOPG, rhRANK, rhTNF RI or rhTNF RII.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.