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Anti-Human Fractalkine (Clone 51637)

Anti-Human Fractalkine (Clone 51637)

Item no.	LEIN-C1405-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Primary Antibodies
Type	Antibody Monoclonal
Applications	FC
Clone	51637
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Patel, DD. et al. (1998) J Exp Med. 188: 1413 2. Hwang, ST. et al. (1999) Eur J Immunol. 29: 2551 3. Muller, GA. et al. (2008) Z Rheumatol. 67: 424 4. Mori, M. et al. (2005) Int J Oncol. 26: 41
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CX3CL1. A suggested method would be to stain cells expressing CX3CL1 with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	Fractalkine
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium1 that mediates attachment and firm adhesion of T cells, monocytes and NK cells.2 FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.3 FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).4
Background	Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium¹ that mediates attachment and firm adhesion of T cells, monocytes and NK cells.² FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.³ FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).⁴
Other Applications Reported In Literature	CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	Fractalkine
Manufacturer - Specificity	Mouse Anti-Human Fractalkine (FKN) (Clone 51637) recognizes an epitope on Human FKN. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2828463
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human FKN (Accession # P78423)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1405-500ug-safety-datasheet.pdf