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Anti-Human CCL16 - 100 µg

Anti-Human CCL16 - 100 µg

Item no.	LEIN-C1481-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Youn, BS. et al. (1998) Biochem. Biophys. Res. Commun. 247(2):217-22. 2. Giovarelli, M. et al. (2006) J. Immunol. 177: 6143 3. Bussolino, F. et al. (2004) Blood 103: 40
NCBI	6360
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	IHC FFPE, N, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	CCL16
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes.1 Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-γ and bacterial lipopolysaccharide. CCL16 is a potent maturation factor for monocyte-derived DCs (MoDCs) through differential use of its four receptors and an indirect regulator of Th cell differentiation.2 CCL16 play a role in hepatic vascular formation during development and in angiogenesis associated to hepatic diseases.3
Background	Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes.¹ Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-γ and bacterial lipopolysaccharide. CCL16 is a potent maturation factor for monocyte-derived DCs (MoDCs) through differential use of its four receptors and an indirect regulator of Th cell differentiation.² CCL16 play a role in hepatic vascular formation during development and in angiogenesis associated to hepatic diseases.³
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human CCL16 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL16 (at 0.75 ng/ml) is 4.0 - 16.0 µg/ml.
PubMed	CCL16
Manufacturer - Research Area	Chemokine
Manufacturer - Specificity	Goat Anti-Human Chemokine (C-C motif) Ligand 16 (CCL16) recognizes Human CCL16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2828547
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	E. coli- Derived Recombinant Human HCC-4
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Chemokine (C-C motif) Ligand 16, LEC, NCC-4, HCC-4, LMC, LCC-1, MTN-1, IL-10-Inducible Chemokine, SCYA16, SCYL4, CKb12, CCL16, Human CCL16, Anti-Human CCL16, C-C motif chemokine 16, CCL16, chemokine (C-C motif) ligand 16, Chemokine CC-4, Chemokine LEC, CKb12, HCC4, HCC-4, HCC-4Liver-expressed chemokine, LCC-1 Lymphocyte and monocyte chemoattractant, LEC, liver CC chemokine-1, LMC, LMCSmall-inducible cytokine A16, monotactin-1, Mtn-1, NCC-4, NCC4IL-10-inducible chemokine, NCC-4ILINCK, new CC chemokine 4, SCYA16MGC117051, SCYL4, small inducible cytokine subfamily A (Cys-Cys), member 16

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1481-100ug-safety-datasheet.pdf