Anti-Human CD20 - Purified - 200 µg

FC, IHC FF, IP

CD20

Next Day 2 - 8°C

CD20 is a 33-37 kD transmembrane-spanning phosphoprotein that facilitates optimal B-cell immune response against T-independent antigens. It can exist in a complex with MHC class I, MHC class II, CD53, CD81, and CD82. However, CD20 can also form homo-oligomers. It has been suggested that homo-oligomerization of CD20 forms calcium ion channels in the plasma membrane of B cells. CD20 can be useful in diagnosing B-cell lymphomas and leukaemias, and is the target of mAbs in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases. Interestingly, studies show a link between the immune system's B cells and diabetes mellitus in which anti-CD20 antibodies rendered the T cell antibodies dysfunctional and, hence, unable to cause insulin desensitivity by a B cell antibody-modulated autoimmune response.

IHC (Frozen) The suggested concentration for this 2h7 antibody in IHC staining on frozen tissue is ≤ 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
IP

Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82

Clone 2H7 recognizes an epitope on human CD20.

0.5 mg/ml

CD20 is present on human pre B lymphocytes and on B lymphocytes, except on plasma cells, and is expressed in tandem with surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.