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Anti-Mouse CD3ε (Clone 500A2) - DyLight® 594 - 100 µg

Anti-Mouse CD3ε (Clone 500A2) - DyLight® 594 - 100 µg

Item no.	LEIN-C1731-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 200 ug 50 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IHC
Clone	500A2
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Syrian
NCBI	12501
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	FC, IHC FF
Manufacturer - Category	Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD3ε
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	This DyLight® 594 conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Background	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Other Applications Reported In Literature	IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
PubMed	CD3ε
Excitation Laser	Red Laser (590 nm)
Ligand/Receptor	Peptide antigen/MHC-complex.
Manufacturer - Research Area	Cell Biology, Immunology, Signal Transduction
Manufacturer - Specificity	Clone 500A2 recognizes an epitope on mouse CD3ε.
RRID	AB_2828795
Concentration	0.2 mg/ml
Formulation	This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Immunogen	Purified Recombinant Mouse CD3ε (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, CD3ε, 500A2, Mouse, Anti-Mouse CD3ε - DyLight® 594

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1731-100ug-safety-datasheet.pdf