Comparison

Anti-Mouse CD25 - DyLight® 650 - 100 µg

Item no. LEIN-C1920-100ug
Manufacturer Leinco Technologies
Amount 100 ug
Quantity options 100 ug 200 ug 50 ug
Category
Type Antibody Monoclonal
Applications FC
Clone PC61
Specific against Mouse (Murine, Mus musculus)
Host Rat
Citations 1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051
2. FW Alt et al. (1995) Immnnity 3: 521
3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S
NCBI 16184
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Shipping Condition Cool pack
Available
Manufacturer - Applications
Quality Tested by Leinco FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category
Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets
CD25
Country of Origin
USA
Shipping Temperature
Next Day 2 - 8°C
Storage Conditions
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.
Background
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.
Additional Reported Applications For Relevant Conjugates
B
Depletion
IHC (Frozen)
IP
For specific conjugates of this clone, review literature for suggested application details.
PubMed
CD25
Excitation Laser
Red Laser (652 nm)
Ligand/Receptor
IL-2
Manufacturer - Research Area
Immunology
Manufacturer - Specificity
Clone PC61 recognizes an epitope on mouse CD25.
RRID
AB_2829000
Concentration
0.2 mg/ml
Formulation
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution
CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords
Monoclonal Antibody, Interleukin-2 Receptor Alpha , IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac, PC61, IL-2 Rα, Mouse, Anti-Mouse IL-2 Rα - DyLight® 650

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

Delivery expected until 12/4/2025 

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