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Anti-Human CD3 [Clone UCHT-1] — DyLight® 594 - 50 µg

Anti-Human CD3 [Clone UCHT-1] — DyLight® 594 - 50 µg

Item no.	LEIN-C2027-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Quantity options	100 ug 200 ug 50 ug
Category	Antibodies Flow Cytometry Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IF
Clone	UCHT-1
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. van Dongen, J. et al. (1988) Blood 71:603 2. Meuer, SC. et al. (1983) Nature 303:808 3. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329 4. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
NCBI	915
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Primary Monoclonal Antibodies>SurfaceTag Human CD Markers
Manufacturer - Targets	CD3
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	This DyLight® 594 conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Background	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Other Applications Reported In Literature	IF
PubMed	CD3
Excitation Laser	Red Laser (590 nm)
Ligand/Receptor	Peptide antigen bound to MHC
Manufacturer - Research Area	Immunology
Manufacturer - Specificity	Clone UCHT-1 recognizes an epitope on human CD3ε.
RRID	AB_2829106
Concentration	0.2 mg/ml
Formulation	This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, CD3, UCHT-1, Human, Anti-Human CD3 - DyLight® 594, cd3, human cd3, cd3 dylight 594

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C2027-50ug-safety-datasheet.pdf