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Anti-Human CD5 (Clone UCHT-2) - DyLight® 550

Anti-Human CD5 (Clone UCHT-2) - DyLight® 550

Item no.	LEIN-C2052-200ug
Manufacturer	Leinco Technologies
Amount	200 ug
Quantity options	100 ug 200 ug 50 ug
Category	Antibodies Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, FA
Clone	UCHT-2
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Leukocyte Typing VI (1995) Oxford University Press 2. Leukocyte Typing III (1987) Oxford University Press 3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids," Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653
NCBI	921
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this UCHT-2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Primary Monoclonal Antibodies>SurfaceTag Human CD Markers
Manufacturer - Targets	CD5
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description	Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.
Background	Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.
Additional Reported Applications For Relevant Conjugates	IHC (Frozen) WB For specific conjugates of this clone, review literature for suggested application details.
PubMed	CD5
Excitation Laser	Yellow Laser (563 nm)
Ligand/Receptor	CD72
Manufacturer - Research Area	Immunology
Manufacturer - Specificity	Mouse Anti-Human CD5 (Clone UCHT-2) recognizes an epitope on Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2829129
Concentration	0.2 mg/ml
Formulation	This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
Immunogen	Purified Recombinant Human CD5 (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C2052-200ug-safety-datasheet.pdf