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Anti-Mouse CD115 (CSF-1R) [Clone AFS98] — Biotin - 100 µg

Anti-Mouse CD115 (CSF-1R) [Clone AFS98] — Biotin - 100 µg

Item no.	LEIN-C2171-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 500 ug
Category	Antibodies Flow Cytometry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC
Clone	AFS98
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Citations	1. Stanley ER., et al. (1982) Cell. 28:71–81 2. Roussel MF., at al. (1988) Cold Spring Harb Symp Quant Biol. 53:521–530 3. Park-Min, KH., et al. (2020) Exp Mol Med 52, 1239–1254 4. Tak, P. P., at al. (2016) Nat. Rev. Drug Disco. 16, 53–70 5. d’Amore F. (2014) Histopathology. 65:490–500 6. Wei YQ., et al. (2012) PLoS One. 7:e50946 7. Rüttinger D., et al. (2017) J Immunother Cancer. 5(1):53
NCBI	12978
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD115
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Product Description	CD115 antibody, clone AFS98, recognizes CD115, also known as mouse colony-stimulating factor 1 receptor (CSF-1R and macrophage colony-stimulating factor receptor (M-CSFR). CD115 is a 150kDa single-pass type I membrane protein encoded by the c-fms gene that belongs to the type III protein tyrosine kinase receptor family. CD115 has an immunoglobulin-like extracellular domain, transmembrane domain, and C-terminal tail receptor and is expressed by monocytes, macrophages, plasmacytoid and conventional dendritic cells (DCs), osteoclasts, and their precursors. CD115 is the receptor for CSF1, also known as M-CSF, and IL-34. Binding and signaling through CD115 regulates the proliferation, differentiation, survival, and cytokine-production of monocytes and macrophages1, 2. In addition, CD115 plays a key role in the differentiation and proliferation of osteoclasts as well as their bone resorption activity3. Mutations in CSF-1R are associated with cancer, including myeloid malignancies, Alzheimer’s disease, and other inflammatory and autoimmune diseases4. The presence of tumor-associated macrophages (TAMs) expressing CSF-1R correlates with poor survival in various tumor types5, 6, and efforts to eliminate these TAMs using CSF1R small-molecule inhibitors and monoclonal antibodies are currently in clinical trials7.
Background	CD115 antibody, clone AFS98, recognizes CD115, also known as mouse colony-stimulating factor 1 receptor (CSF-1R and macrophage colony-stimulating factor receptor (M-CSFR). CD115 is a 150kDa single-pass type I membrane protein encoded by the c-fms gene that belongs to the type III protein tyrosine kinase receptor family. CD115 has an immunoglobulin-like extracellular domain, transmembrane domain, and C-terminal tail receptor and is expressed by monocytes, macrophages, plasmacytoid and conventional dendritic cells (DCs), osteoclasts, and their precursors. CD115 is the receptor for CSF1, also known as M-CSF, and IL-34. Binding and signaling through CD115 regulates the proliferation, differentiation, survival, and cytokine-production of monocytes and macrophages^{1, 2}. In addition, CD115 plays a key role in the differentiation and proliferation of osteoclasts as well as their bone resorption activity³. Mutations in CSF-1R are associated with cancer, including myeloid malignancies, Alzheimer’s disease, and other inflammatory and autoimmune diseases⁴. The presence of tumor-associated macrophages (TAMs) expressing CSF-1R correlates with poor survival in various tumor types^{5, 6}, and efforts to eliminate these TAMs using CSF1R small-molecule inhibitors and monoclonal antibodies are currently in clinical trials⁷.
Additional Reported Applications For Relevant Conjugates	B FA For specific conjugates of this clone, review literature for suggested application details.
PubMed	CD115
Ligand/Receptor	Macrophage colony stimulating factor (M-CSF), IL-34
Manufacturer - Research Area	Immunology
Manufacturer - Specificity	Clone AFS98 recognizes an epitope on mouse CD115.
RRID	AB_2829255
Concentration	0.5 mg/ml
Formulation	This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	CD115 is expressed on monocytes, macrophages, plasmacytoid and conventional dendritic cells, osteoclasts, and peritoneal exudate cells.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Macrophage Colony Stimulating Factor Receptor, CD115, M-CSFR, CSF-R1, C-fms, AFS98, M-CSFR, Mouse, Anti-Mouse CD115 (CSF-1R) - Biotin, Flow Cytometry

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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LEIN-C2171-100ug-safety-datasheet.pdf