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Anti-Mouse CD1d (Clone D5) - Purified In vivo GOLD™ Functional Grade

Anti-Mouse CD1d (Clone D5) - Purified In vivo GOLD™ Functional Grade

Item no.	LEIN-C2451-50mg
Manufacturer	Leinco Technologies
Amount	50 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	In Vivo Antibodies In Vivo Antibodies Immunoprecipitation Western Blot
Type	Antibody Monoclonal
Applications	WB, IP, IV
Clone	D5
Specific against	Human (Homo sapiens)
Host	Mouse
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
NCBI	912
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco WB The suggested concentration for this CD1d (clone D5) antibody for use in western blotting is 1-10 μg/ml.
Manufacturer - Targets	CD1d
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Background	CD1d antibody, D5, recognizes human CD1d, also known as CD1.1 and Ly-38, a 48 kDa type 1 membrane glycoprotein that belongs to the cluster of differentiation 1 (CD1) family of glycoproteins. CD1d is expressed on epithelial cells and monocyte-derived antigen presenting cells (APCs), including monocytes, DCs, and macrophages^{1, 2}. CD1d is an MHC class I-like molecule involved in the presentation of lipid antigens, such as α-galactosylceramide (α-GalCer), to a highly conserved T cell subset, named invariant natural killer T (iNKT) cells or CD1d-restricted T cells¹. Following activation, iNKT cells rapidly proliferate and produce large amounts of cytokines and cytolytic mediators^{3, 4}.
Other Applications Reported In Literature	IP
PubMed	CD1d
Manufacturer - Research Area	Apoptosis, Immunology
Manufacturer - Specificity	Clone D5 recognizes an epitope on human CD1d.
RRID	AB_2893555
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD1d is expressed on monocytes, DCs, macrophages, and epithelial cells.
Endotoxin Level	≤ 1.0 EU/mg as determined by the LAL method
Immunogen	CD1d a1-a2 domains
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C2451-50mg-safety-datasheet.pdf