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Anti-Human CD62L - Purified <i>in vivo </i>PLATINUM™ Functional Grade - 100 mg

Anti-Human CD62L - Purified <i>in vivo </i>PLATINUM™ Functional Grade - 100 mg

Item no.	LEIN-C6270-100mg
Manufacturer	Leinco Technologies
Amount	100 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IF, IHC, other, IV
Clone	DREG56
Specific against	Human (Homo sapiens)
Host	Mouse
Purity	≥98% monomer by analytical SEC, >95% by SDS Page
NCBI	6402
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	B, FC, IF, IHC FF, in vivo, PhenoCycler®, WB
Manufacturer - Category	PhenoCycler®\|Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>SurfaceTag Human CD Markers
Manufacturer - Targets	CD62L
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes1. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition2, 3. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)1. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation4.
Background	CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes¹. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition^{2, 3}. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)¹. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation^{4^.}
Pathogen Testing	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Function	Leukocyte homing, leukocyte tethering, rolling
Additional Reported Applications For Relevant Conjugates	CODEX®
Other Applications Reported In Literature	IHC FF IF B
PubMed	CD62L
Ligand/Receptor	CD34, GlyCAM, MAdCAM-1
Manufacturer - Research Area	Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Manufacturer - Specificity	Clone DREG56 recognizes an epitope on human CD62L.
RRID	AB_2829786
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD62L is expressed on most circulating leukocytes, including neutrophils, monocytes, T, B and NK cells, and human embryo trophoblasts.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, L-Selectin, Mel-14, LAM-1, LEU-8, DREG56, CD62L, Human CD62L, Anti-Human CD62L, MEL14, DREG-56. Clone DREG56, CD62L antigen, CD62L, gp90-MEL, hLHRc, LAM1, LAM-1, LAM1LECAM1, LECAM1, LEU8, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LNHRTQ1, LSEL, L-Selectin, LYAM1, Lyam-1, LYAM1CD62 antigen-like family member L, Lymph node homing receptor, lymphocyte adhesion molecule 1, pln homing receptor, PLNHR, selectin L, SELL, TQ1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C6270-100mg-safety-datasheet.pdf