« Back
Home /
Anti-Human/Mouse CDCP1 (CD318) - Purified in vivo PLATINUM™ Functional Grade

Anti-Human/Mouse CDCP1 (CD318) - Purified in vivo PLATINUM™ Functional Grade

Item no.	LEIN-C9105-1mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 5 mg
Category	In Vivo Antibodies Primary Antibodies
Type	Antibody Primary
Applications	FC, IV
Clone	9A2
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Mouse
Purity	≥98% monomer by analytical SEC, >95% by SDS Page
Citations	1. Moasser, MM. et al. (2005) Oncogene. 24(34):5333-43. 2. Moasser, MM. et al. (2013) Cancer Res. 73(3):1168-79. 3. Lin, F. et al. (2017) Proc Natl Acad Sci U S A. 114(33):E6912-21.
NCBI	64866
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this 9A2 antibody for staining cells in flow cytometry is 1 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Primary Monoclonal Antibodies>Immune Checkpoints\|Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD318
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CDCP1 is a transmembrane glycoprotein of molecular weight 140 kDa. CDCP1 is a ligand for a receptor molecule (CD6) that is expressed on some T-cells and is thought to be involved in T-cell migration and chemotaxis. It has a large extracellular domain that includes two CUB domains, and a smaller intracellular domain. The smaller intracellular domain contains five tyrosine residues (Y707, Y734, Y743, Y762 and Y806). CDCP1 is cleaved next to Arg368 (at the extracellular domain) by serine proteases. This cleavage yields a truncated molecule with a molecular weight of 80 kDa.1 CDCP1 is not typically cleaved In vivo. However, its cleavage can be induced during tumorigenesis or tissue injury.2 CDCP1 acts as a substrate for Src family kinases which exclusively mediate the phosphorylation of CDCP1. In cultured cells, tyrosine phosphorylation of CDCP1 transpires when cells are stimulated to detach via trypsin or EDTA. This detachment is associated with the phosphorylation of CDCP1 along with the simultaneous dephosphorylation of focal adhesion proteins. Conversely, during cellular attachment, CDCP1 is dephosphorylated, while focal adhesion proteins are simultaneously phosphorylated. Furthermore, CDCP1 is suspected to play a role in autoimmune diseases such as encephalomyelitis, multiple sclerosis and inflammatory arthritis.3
Background	CDCP1 is a transmembrane glycoprotein of molecular weight 140 kDa. CDCP1 is a ligand for a receptor molecule (CD6) that is expressed on some T-cells and is thought to be involved in T-cell migration and chemotaxis. It has a large extracellular domain that includes two CUB domains, and a smaller intracellular domain. The smaller intracellular domain contains five tyrosine residues (Y707, Y734, Y743, Y762 and Y806). CDCP1 is cleaved next to Arg368 (at the extracellular domain) by serine proteases. This cleavage yields a truncated molecule with a molecular weight of 80 kDa.¹ CDCP1 is not typically cleaved In vivo. However, its cleavage can be induced during tumorigenesis or tissue injury.² CDCP1 acts as a substrate for Src family kinases which exclusively mediate the phosphorylation of CDCP1. In cultured cells, tyrosine phosphorylation of CDCP1 transpires when cells are stimulated to detach via trypsin or EDTA. This detachment is associated with the phosphorylation of CDCP1 along with the simultaneous dephosphorylation of focal adhesion proteins. Conversely, during cellular attachment, CDCP1 is dephosphorylated, while focal adhesion proteins are simultaneously phosphorylated. Furthermore, CDCP1 is suspected to play a role in autoimmune diseases such as encephalomyelitis, multiple sclerosis and inflammatory arthritis.³
Pathogen Testing	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
PubMed	CD318
Manufacturer - Specificity	Clone 9A2 recognizes an epitope within the extracellular domain of human/mouse CDCP1.
RRID	AB_2829877
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CDCP1 is widely expressed in the esophagus, skin, colon, duodenum, stomach and other tissues.
Endotoxin Level	< 0.5 EU/mg as determined by the LAL method
Immunogen	His-tagged recombinant fragment from the internal region of human CUB domain-containing protein 1
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

LEIN-C9105-1mg-safety-datasheet.pdf