Anti-Mouse CCL21 - 25 µg

Item no.	LEIN-E115-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug
Category	Neuroscience Neuroinflammation Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB, FC, IHC
Specific against	Mouse (Murine, Mus musculus)
Host	Caprine
NCBI	18829
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, FC, IHC, N, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	CCL21
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7. In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity.
Background	Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7. In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity.
Other Applications Reported In Literature	Neutralization: This antibody is useful for neutralization of Mouse CCL21 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CCL21 (at 50 ng/ml) is 0.5 - 2.5 µg/ml. Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	CCL21
Manufacturer - Research Area	Chemokine
Manufacturer - Specificity	Goat Anti-Mouse CCL21 recognizes Mouse CCL21. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2829972
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Mouse CCL21 (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Chemokine C-C Motif Ligand 21, 6Ckine, CKb9, ECL, MGC34555, SCYA21, SLC, TCA4, Exodus-2, CCL21, Mouse CCL21, Anti-Mouse CCL21, 6Ckine, 6CkineSmall-inducible cytokine A21, Beta-chemokine exodus-2, CCL21, chemokine C-C motif ligand 21, CKb9, exodus-2, member 21, SCYA21, SCYA21MGC34555, secondary lymphoid tissue chemokine, SLC, SLCSecondary lymphoid-tissue chemokine, TCA-4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-E115-25ug-safety-datasheet.pdf