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Anti-Human Ephrin-A4 (Clone 135305) - 25 µg

Anti-Human Ephrin-A4 (Clone 135305) - 25 µg

Item no.	LEIN-E248-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	25 ug 500 ug
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, IHC
Clone	135305
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Logtenberg, T. et al. (2000) Blood 95: 221
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Human Ephrin-A4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Ephrin-A4 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E199.
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	Ephrin-A4
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Ephrin-A4 is a glycoinositol phospholipid membrane anchor containing ephrin found in variety of adult tissue such as kidney, lung, colon and ovary. Ephrin-A4 is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases.1 Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-A4 encodes an EFNA class ephrin. Ephrin-A4 (s) may play a role in the interaction between activated B lymphocytes and dendritic cells in human tonsils.
Background	Ephrin-A4 is a glycoinositol phospholipid membrane anchor containing ephrin found in variety of adult tissue such as kidney, lung, colon and ovary. Ephrin-A4 is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases.¹ Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-A4 encodes an EFNA class ephrin. Ephrin-A4 (s) may play a role in the interaction between activated B lymphocytes and dendritic cells in human tonsils.
Other Applications Reported In Literature	Immunohistochemistry: Suitable for use at concentration of 5-25 µg/mL.
PubMed	Ephrin-A4
Manufacturer - Specificity	Mouse Anti-Human Ephrin-A4 (Clone 135305) recognizes an epitope on Human Ephrin-A4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2830020
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human Ephrin-A4 (Accession # P52798)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Ephrin-A4, LERK-4, EFL-4, EFNA4, EPLG4, MGC125826, 135305, Human, Anti-Human Ephrin-A4, EFL4, EFL-4, EFNA4, EPH-related receptor tyrosine kinase ligand 4, EphrinA4, Ephrin-A4, EPLG4MGC125826, LERK-4, LERK4FLJ57652, ligand of eph-related kinase 4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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LEIN-E248-25ug-safety-datasheet.pdf