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Anti-Mouse Ephrin-A2

Anti-Mouse Ephrin-A2

Item no.	LEIN-E263-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC, other
Specific against	Mouse (Murine, Mus musculus)
Host	Caprine
Citations	1. Xie, D. et al. (2007) Int J Oncol. 30: 865 2. Frisén, J. et al. (2005) Genes Dev. 19: 462 3. Hejtmancik, JF. et al. (2008) Mol Vis. 14: 2042 4. Debinski, W. et al. (2005) Mol Cancer Res. 3: 541
NCBI	13637
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Mouse Ephrin-A2 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Ephrin-A2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	EphA2
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Ephrin-A2 (EPHA2), also known as EFNA2 is an oncoprotein1 that is a member of the ephrin ligand family, which binds members of the Eph receptor family. Ephrin-A2 is a negative regulator of proliferation.2 EPHA2 plays a vital role in maintaining lens transparency.3 It also represents a new marker and novel target for the development of molecular therapeutics against glioblastoma multiforme (GBM).4
Background	Ephrin-A2 (EPHA2), also known as EFNA2 is an oncoprotein¹ that is a member of the ephrin ligand family, which binds members of the Eph receptor family. Ephrin-A2 is a negative regulator of proliferation.² EPHA2 plays a vital role in maintaining lens transparency.³ It also represents a new marker and novel target for the development of molecular therapeutics against glioblastoma multiforme (GBM).⁴
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/mL. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Blocking: Approximately 1 - 5 μg/mL of this antibody will block 50% of the binding of 10 ng/mL of rmEphrin-A2/Fc to immobilized rrEph-A5/Fc (100 μL of a 2 μg/mL solution was coated in each well) in a functional ELISA.
PubMed	EphA2
Manufacturer - Specificity	Goat Anti-Mouse Ephrin-A2 recognizes Mouse Ephrin-A2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2830035
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	NS0-Derived Recombinant Mouse Ephrin-A2 Extracellular Domain
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-E263-100ug-safety-datasheet.pdf