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Anti-Human Fibulin-4 - Purified <i>in vivo </i>PLATINUM™ Functional Grade - 100 mg

Anti-Human Fibulin-4 - Purified <i>in vivo </i>PLATINUM™ Functional Grade - 100 mg

Item no.	LEIN-F2205-100mg
Manufacturer	Leinco Technologies
Amount	100 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	In Vivo Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, IF, IHC
Clone	5G11
Specific against	Human (Homo sapiens)
Host	Mouse
Purity	≥98% monomer by analytical SEC, >95% by SDS Page
Citations	1. DiCorleto, PE. et al. (2009) Biochem J. 423(1): 79–89. 2. Kobayashi, N. et al. (2007) J Biol Chem. 282(16):11805-16. 3. Gallagher, WM. et al. (1999) Oncogene. 18(24):3608-16. 4. Gallagher, WM. et al. (2001) FEBS Lett. 489(1):59-66.
NCBI	30008
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	IF Microscopy, IF Staining, IHC FFPE, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Immune Checkpoints\|Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	Fibulin-4
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	There are currently seven genes in the fibulin family (Fibulin-1, 2, 3, 4, 5, 6, and 7) that are associated with elastic fibers. The fibulins are widely distributed throughout the body and are more prominent in tissues rich in elastic fibers and are often associated with vasculature and elastic tissues. Fibulin-4 appears to be the most critical member of this family of proteins in modulating elastic-fiber formation because studies have shown that fibulin-4-null mice do not produce elastic fibers at all.1 This extracellular glycoprotein has a molecular weight of approximately 49 kD, and is not only considered a structural protein of the extracellular matrix, but also a regulatory factor required for normal elastin expression in human fibroblasts.2 Due to the regulatory role of Fibulin-4, it is thought that a pathophysiological link may exist between the modulation of fibulin-4 levels and diseases, such as WBS (Williams-Beuren syndrome), that are characterized by impaired elastic-fiber formation.1 Furthermore, fibulin-4 has been shown to interact with P53, a crucial homologous protein that prevents cancer formation, and thus functions as a tumor suppressor.3 One study indicated that colon tumors were found to have approximately 2-7-fold increases of fibulin-4 mRNA expression. This suggests that the dysregulated expression of the fibulin-4 gene is associated with human colon tumourigenesis.4
Background	There are currently seven genes in the fibulin family (Fibulin-1, 2, 3, 4, 5, 6, and 7) that are associated with elastic fibers. The fibulins are widely distributed throughout the body and are more prominent in tissues rich in elastic fibers and are often associated with vasculature and elastic tissues. Fibulin-4 appears to be the most critical member of this family of proteins in modulating elastic-fiber formation because studies have shown that fibulin-4-null mice do not produce elastic fibers at all.¹ This extracellular glycoprotein has a molecular weight of approximately 49 kD, and is not only considered a structural protein of the extracellular matrix, but also a regulatory factor required for normal elastin expression in human fibroblasts.² Due to the regulatory role of Fibulin-4, it is thought that a pathophysiological link may exist between the modulation of fibulin-4 levels and diseases, such as WBS (Williams-Beuren syndrome), that are characterized by impaired elastic-fiber formation.¹ Furthermore, fibulin-4 has been shown to interact with P53, a crucial homologous protein that prevents cancer formation, and thus functions as a tumor suppressor.³ One study indicated that colon tumors were found to have approximately 2-7-fold increases of fibulin-4 mRNA expression. This suggests that the dysregulated expression of the fibulin-4 gene is associated with human colon tumourigenesis.⁴
Pathogen Testing	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Other Applications Reported In Literature	IF Staining IF Microscopy IHC FFPE
PubMed	Fibulin-4
Manufacturer - Research Area	Immunology, Stem Cell
Manufacturer - Specificity	Clone 5G11 recognizes Human Fibulin-4. Monoclonal antibodies raised against the purified proteins did not show any crossreactivity with other family members.
RRID	AB_2830197
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Fibulin-4 is predominantly expressed in tissues rich in elastic fibers.
Immunogen	Recombinant, human Fibulin-4 produced in E. coli BL21 cells.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	EFEMP2, ARCL1B, FBLN4, MBP1, UPH1, EGF containing fibulin like extracellular matrix protein 2, EGF containing fibulin extracellular matrix protein 2; FIBL-4; fibulin 4; mutant p53 binding protein 1

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-F2205-100mg-safety-datasheet.pdf