Anti-Human GFRalpha-3

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Sf 21-Derived Recombinant Human GFRα-3 (Accession # AAC24355)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830266

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human GFRα-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human GFRα-3 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Glial Cell Line-Derived Neurotropic Factor Receptor Alpha 3 (GFRα-3) (Clone 111004) recognizes an epitope on Human GFRα-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows approximately 20% cross-reactivity with rmGFRα-3 and no cross-reactivity with rhGFRα-1 or GFRα-4.

Background

GDNF family receptor alpha 3, also known as GFRA3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. GFRalpha3 preferentially binds the newest member of the glial-derived neurotrophic factor family of ligands, artemin. The major site of GFRalpha3 expression is in the dorsal root ganglion. GFRalpha3 artemin neurons are immunopositive for markers expected of nociceptors and include a subset of neurons distinct from the GDNF-responsive population.¹

Antigen Details

Protein

GDNF R alpha-3

PubMed

GDNF R alpha-3

References & Citations

1. Sanicola, M. et al. (2001)Eur J Neurosci 13: 2177

Technical Protocols