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Anti-Human GFRα-3 - 25 µg

Anti-Human GFRα-3 - 25 µg

Item no.	LEIN-G677-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	25 ug 500 ug
Category	Neuroscience Neural Signaling Antibodies Western Blot Primary Antibodies
Type	Antibody Monoclonal
Applications	WB
Clone	111004
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Sanicola, M. et al. (2001)Eur J Neurosci 13: 2177
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Human GFRα-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human GFRα-3 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	GDNF R alpha-3
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	GDNF family receptor alpha 3, also known as GFRA3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. GFRalpha3 preferentially binds the newest member of the glial-derived neurotrophic factor family of ligands, artemin. The major site of GFRalpha3 expression is in the dorsal root ganglion. GFRalpha3 artemin neurons are immunopositive for markers expected of nociceptors and include a subset of neurons distinct from the GDNF-responsive population.1
Background	GDNF family receptor alpha 3, also known as GFRA3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. GFRalpha3 preferentially binds the newest member of the glial-derived neurotrophic factor family of ligands, artemin. The major site of GFRalpha3 expression is in the dorsal root ganglion. GFRalpha3 artemin neurons are immunopositive for markers expected of nociceptors and include a subset of neurons distinct from the GDNF-responsive population.¹
PubMed	GDNF R alpha-3
Manufacturer - Specificity	Mouse Anti-Human Glial Cell Line-Derived Neurotropic Factor Receptor Alpha 3 (GFRα-3) (Clone 111004) recognizes an epitope on Human GFRα-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows approximately 20% cross-reactivity with rmGFRα-3 and no cross-reactivity with rhGFRα-1 or GFRα-4.
RRID	AB_2830266
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Sf 21-Derived Recombinant Human GFRα-3 (Accession # AAC24355)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Glial Cell Line-Derived Neurotropic Factor Receptor Alpha 3, GDNFRγ, GDNFR Gamma, 111004, GFRα-3, Human, Anti-Human GFRα-3, GDNF family receptor alpha 3, GDNF family receptor alpha-3, GDNF R alpha-3, GDNF receptor alpha-3, GDNFR-alpha-3, GFR alpha3, GFR alpha-3, GFRA3, GFRa-3, GFR-alpha-3, glial cell line-derived neurotrophic factor receptor alpha-3, GPI-linked receptor

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LEIN-G677-25ug-safety-datasheet.pdf