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Anti-Human/Mouse Integrin β7 - FITC - 500 µg

Anti-Human/Mouse Integrin β7 - FITC - 500 µg

Item no.	LEIN-I-1142-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Quantity options	500 ug 50 ug
Category	Antibodies Flow Cytometry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC
Clone	FIB504
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
NCBI	16421
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco Flow Cytometry: For flow cytometric staining using the µg sizes, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. Each lot of this antibody is quality control tested.
Manufacturer - Category	Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	Integrin Beta 7
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	This monoclonal antibody FITC conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description	Integrin β7 antibody, clone FIB504, recognizes integrin β7, a 130 kDa membrane glycoprotein of the Ig superfamily. Integrin β7 forms heterodimers with both the α4 (CD49d) and aE (CD103) integrins. α4β7 integrin is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors1, 2. It mediates adhesion to mucosal endothelial cells, promoting leukocyte transendothelial migration through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1)3, 4. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells5, 6, subsets of dendritic cells7, and regulatory T cells (Tregs)8. It facilitates retention in the gut epithelial layer via interactions with E-cadherin9. Anti-β7 antibodies block both the homing to and retention in the gut of pathogenic T cells10 and are currently under evaluation in a phase 3 clinical trial to treat inflammatory bowel disease11.
Background	Integrin β7 antibody, clone FIB504, recognizes integrin β7, a 130 kDa membrane glycoprotein of the Ig superfamily. Integrin β7 forms heterodimers with both the α4 (CD49d) and aE (CD103) integrins. α4β7 integrin is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors^{1, 2}. It mediates adhesion to mucosal endothelial cells, promoting leukocyte transendothelial migration through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1)^{3, 4}. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells^{5, 6}, subsets of dendritic cells⁷, and regulatory T cells (Tregs)8. It facilitates retention in the gut epithelial layer via interactions with E-cadherin⁹. Anti-β7 antibodies block both the homing to and retention in the gut of pathogenic T cells¹⁰ and are currently under evaluation in a phase 3 clinical trial to treat inflammatory bowel disease¹¹.
Function	Lymphocyte adhesion, hematopoietic progenitor cells migration
Other Applications Reported In Literature	B This antibody has been reported to block beta7 integrin mediated cell adhesion for In vitro and In vivo studies. Additional reported applications (for the relevant formats) include: Western Blotting Immunoprecipitation
PubMed	Integrin Beta 7
Excitation Laser	Blue Laser (490 nm)
Ligand/Receptor	CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Manufacturer - Research Area	Cell Biology, Immunology, Neuroscience
Manufacturer - Specificity	Clone FIB504 recognizes an epitope on human/mouse integrin β7.
RRID	AB_2830481
Concentration	0.2 mg/ml
Formulation	This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation	This monoclonal antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Antigen Distribution	Integrin α4β7 is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells, subsets of dendritic cells, and regulatory T cells (Tregs)8.
Immunogen	TK1 Cells
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Integrin Beta 7, FIB504, Integrin β7, Mouse, Anti-Mouse/Human Integrin β7 - FITC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-1142-500ug-safety-datasheet.pdf