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Anti-Mouse IFNß [Clone HDß-4A7] — Purified <i>in vivo </i>GOLD™ Functional Grade - 25 mg

Anti-Mouse IFNß [Clone HDß-4A7] — Purified <i>in vivo </i>GOLD™ Functional Grade - 25 mg

Item no.	LEIN-I-1182-25mg
Manufacturer	Leinco Technologies
Amount	25 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies Western Blot Primary Antibodies Functional Assays
Type	Antibody Monoclonal
Applications	WB, FA, other, IV
Clone	HDß-4A7
Specific against	Mouse (Murine, Mus musculus)
Host	Mouse
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
NCBI	3456
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	B, FA, in vivo, N, WB
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Growth Factors and Cytokines\|Purified in vivo Functional Grade
Manufacturer - Targets	IFNβ
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Other Applications Reported In Literature	FA B N WB In Vitro Activity of HDß-4A7 - Inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). Blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. Neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV. In Vivo Activity of HDß-4A7 circulates with a half-life of ~ 14 days. Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice.
PubMed	IFNβ
Manufacturer - Research Area	Apoptosis, Cell Biology, Cell Death, Signal Transduction, Tumor Suppressors
Manufacturer - Specificity	Mouse Anti-Mouse Interferon-beta (IFN-ß) (Clone HDß-4A7) recognizes an epitope on Mouse IFN-ß and binds specifically to murine IFNß and does not bind murine IFNg or several different IFNa species (a-1, 4, 5 or 13); HDb-4A7 binds both recombinant and natural forms. This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2737532
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Immunogen	Plasmid DNA encoding murine IFNb
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Interferon beta, IFNb, IFN-beta, Type-1 interferon, HDß-4A7, IFN-ß, Mouse IFNb, HDbeta-4A7, IFN beta, HDb-4A7, rmIFN-β, Fibroblast interferon, IFBIFNB, IFF, IFNB1, IFNbeta, IFN-beta, interferon beta, interferon beta 1 fibroblast, MGC96956, HDβ-4A7

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-1182-25mg-safety-datasheet.pdf