Anti-Human CD307e (FcRL5) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Cells transfected with Human FcRL5.

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2892927

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this CD307e antibody for staining cells in flow cytometry is ≤ 0.125 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

B
IP

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 50946 recognizes human FcRL5 within the first 3 Ig domains. Clone 50946 does not cross-react with FcRL4.

Antigen Distribution

FcRL5 is expressed on mature B cells and plasma cells.

Background

FcRL5 antibody, 509F6, recognizes Fc receptor-like 5 (FcRL5), also known as immunoglobulin superfamily receptor translocation associated 2 (IRTA2), Fc receptor homolog 5 (FcRH5), and BXMAS1. FcRL5 is a 106 kDa type I transmembrane glycoprotein containing 9 Ig-like extracellular domains, a cytoplasmic ITAM-like consensus sequence, and two cytoplasmic ITIM-like sequences¹. FcRL5 is found on most mature B cells, with the highest levels on naive and memory B cells and plasma cells^{1, 2}. FcRL5 is an IgG receptor and binds to all IgG isotypes³, resulting in the inhibition of BCR signaling through the recruitment of SH2 domain-containing tyrosine phosphatase 1 (SHP-1) after tyrosine phosphorylation of its two ITIMs⁴. B cell malignancies, including multiple myeloma, chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia, often exhibit aberrant FCRL5 expression, indicating FcRL5 as a potential biomarker or therapeutic target^5-7.

Antigen Details

Protein

FcRL5

Ligand/Receptor

Aggregated IgG

PubMed

FcRL5

NCBI Gene Bank ID

83416

UniProt.org

Information on Uniprot.org

References & Citations

1. M. D. Cooper., et al. (2001). Proc. Natl. Acad. Sci. USA 98: 9772–9777

2. Koeppen H., et al. (2006) Int Immunol. 18:1363-1373

3. Colonna M., et al. (2012) J Immunol. 188(10):4741-5

4. M. D. Cooper., et al (2007) Proc. Natl. Acad. Sci. USA 104: 9770–9775

5. Dalla-Favera R., et al. (2001) Immunity. 14(3):277-89

6. Pastan I., et al. (2007) Leukemia. 21(1):169-74

7. Nagata S., et al. (2005) Clin Cancer Res. 11(1):87-96

Technical Protocols