Anti-Human IL-2Rα - 500 µg

ICC, IHC FFPE, N, WB

IL-2 R alpha

Next Day Ambient

IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation.1 IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon activation of mature lymphocytes. IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation.2 IL-2Ralpha is also important in the formation of high-affinity binding sites, and the inducibility of the alpha gene contributes to the highly regulated and transient display of high-affinity IL-2R.3

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human IL-2R α bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-2R α (at 1 ng/ml) is 0.5-1.0 µg/ml.
Immunocytochemistry: Suitable for use at concentration of8-25 µg/mL.

Mouse Anti-Human Interleukin-2 Receptor alpha (IL-2Rα) (Clone 22722) recognizes an epitope on Human IL-2Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.