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Anti-Human IL-12 (Clone 24910) - 100 µg

Anti-Human IL-12 (Clone 24910) - 100 µg

Item no.	LEIN-I-351-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Western Blot Immunocytochemistry Primary Antibodies Immunochemistry
Type	Antibody Monoclonal
Applications	WB, ICC
Clone	24910
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28 2. Ritz, J. et al. (2001) Blood. 97(12):3860-6. 3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	ICC, N, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	IL-12
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody should be stored desiccated at -20°C to -70°C. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	IL-12 plays a role in resistance against pathogens via the differentiation of naive T cells into Th1 cells. It stimulates the growth and function of T cells, blocks formation of new blood vessels, and contributes to antimycobacterial immune response. In addition, it promotes the production of IFN-γ and TNF-α and reduces IL-4 mediated suppression of IFN-γ. Consequently, this enhances the immunostimulatory and immunomodulatory effects of IFN-γ. In addition, there appears to be a link between IL-2 and the signal transduction of IL-12 in NK cells, which enhances the functional response of IL-12 via IFN-γ production and killing of target cells. Furthermore, IL-12 is thought to be associated with autoimmunity. IL-12 was shown to worsen the condition when administered to people already suffering from autoimmune diseases. Comparatively, inhibition of IL-12 (either through IL-12 gene knock-out in mice or treatment of mice with IL-12 mAbs) improved the disease.
Background	IL-12 plays a role in resistance against pathogens via the differentiation of naive T cells into Th1 cells. It stimulates the growth and function of T cells, blocks formation of new blood vessels, and contributes to antimycobacterial immune response. In addition, it promotes the production of IFN-γ and TNF-α and reduces IL-4 mediated suppression of IFN-γ. Consequently, this enhances the immunostimulatory and immunomodulatory effects of IFN-γ. In addition, there appears to be a link between IL-2 and the signal transduction of IL-12 in NK cells, which enhances the functional response of IL-12 via IFN-γ production and killing of target cells. Furthermore, IL-12 is thought to be associated with autoimmunity. IL-12 was shown to worsen the condition when administered to people already suffering from autoimmune diseases. Comparatively, inhibition of IL-12 (either through IL-12 gene knock-out in mice or treatment of mice with IL-12 mAbs) improved the disease.
Other Applications Reported In Literature	Neutralization: Human IL-12 stimulates the ³H-thymidine incorporation by PHA activated human peripheral blood mononuclear cells in a dose-dependent manner¹. The ED₅₀ for this effect is typically 0.05 - 0.2 ng/mL. This antibody is useful for neutralization of Human IL-12 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-12 (at 1.0 ng/ml) is 0.3 - 0.9 µg/ml. The bioassay was perfromed using PHA-activated peripheral blood mononuclear cell (PBMC) proliferation assay. Immunocytochemistry: This antibody can be used at 8 - 25 μg/mL with the appropriate secondary reagent such as PN:M103 to detect human IL-12 in cultured cells or tissue sections using PN:K107 or PN:D100.
PubMed	IL-12
Manufacturer - Research Area	Other Molecules
Manufacturer - Specificity	Clone 24910 recognizes an epitope on human IL-12.
RRID	AB_2830645
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg
Immunogen	Purified Recombinant Sf 21-derived rhIL-12 (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Interleukin-12, NKSF, TSF, Maturation Factor, Cytotoxic Lymphocyte Maturation Factor (CLMF), p35, 24910, IL-12, Human, Anti-Human IL-12, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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LEIN-I-351-100ug-safety-datasheet.pdf