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Anti-Human IL-4 R alpha - 25 µg

Anti-Human IL-4 R alpha - 25 µg

Item no.	LEIN-I-638-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunocytochemistry Primary Antibodies Immunochemistry
Type	Antibody Monoclonal
Applications	WB, FC, IHC, ICC
Clone	25463
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Raine, CS. et al. (2008) Am J Pathol. 173(1):119-29. 2. Puri, RK. et al. (2001) Cancer Res. 61: 8058 3. Jiang, H. et al. (2000) J. Allergy Clin. Immunol. 105:1063 4. Hall, IP. et al. (2000) Respir. Res. 1:6 5. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, FC, ICC, IHC FFPE, N, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	IL-4 R alpha
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Interleukin 4 receptor (IL-4R) is a type I cytokine receptor that is expressed by lymphocytes and is well known for its role in immunomodulatory signaling and has also been documented on oligodendrocytes, suggesting involvement in glial cell interactions. IL-4R exerts a negative regulatory role on oligodendrocytes that when deleted results in enhanced myelin repair.1 IL-4R in brain tumors may serve as an attractive target for anticancer therapy.2
Background	Interleukin 4 receptor (IL-4R) is a type I cytokine receptor that is expressed by lymphocytes and is well known for its role in immunomodulatory signaling and has also been documented on oligodendrocytes, suggesting involvement in glial cell interactions. IL-4R exerts a negative regulatory role on oligodendrocytes that when deleted results in enhanced myelin repair.¹ IL-4R in brain tumors may serve as an attractive target for anticancer therapy.²
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8-25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human IL-4R bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-4R (at 0.2 ng/ml) is 3-6 µg/ml. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	IL-4 R alpha
Manufacturer - Specificity	Mouse Anti-Human Interleukin 4 Receptor (IL-4R) (Clone 25463) recognizes an epitope on Human IL-4R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhIL-9 R, rmIL-4 R, rhIL-5 Rα, rhIL-5 Rβ, rhIL-13 Rα1 or rhIL-13 Rα2
RRID	AB_2830760
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human IL-4 R (Accession # P24394)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Interleukin-4 Receptor, CD124, IL4RA, 25463, IL-4 R, Human, Anti-Human IL-4 Receptor, CD124 antigen, CD124, IL-4 R alpha, IL-4 receptor subunit alpha, IL4R alpha, IL-4R subunit alpha, IL4R, IL-4Ra, IL4RACD124, IL-4R-alpha, interleukin 4 receptor, interleukin-4 receptor alpha chain, interleukin-4 receptor subunit alpha

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-638-25ug-safety-datasheet.pdf