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Anti-Human IFN-γ - 100 µg

Anti-Human IFN-γ - 100 µg

Item no.	LEIN-I-646-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Immunocytochemistry Primary Antibodies Immunochemistry
Type	Antibody Monoclonal
Applications	ICC
Clone	25718
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Goeddel, DV. et al. (1982) Nature 298: 859 2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41 3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	ICC, ICFC, N
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	IFNγ
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
Background	Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.¹ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.² IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.³
Other Applications Reported In Literature	NThis antibody is useful for neutralization of Human IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IFN-γ (at 50 ng/ml) is 0.02 - 0.06 µg/ml. ICC Immunocytochemistry suitable for use at concentration of 8-25 µg/mL.
PubMed	Interferon Gamma
Manufacturer - Research Area	Other Molecules
Manufacturer - Specificity	Mouse Anti-Human Interferon Gamma (IFN-γ) (Clone 25718) recognizes an epitope on Human IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2830768
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human IFN-γ (Accession # AAP20098.1)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Interferon Gamma, Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI, 25718, IFN-γ, Human, Anti-Human IFN-γ, IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-646-100ug-safety-datasheet.pdf