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Anti-Human IL-3 Rα (CD123) - 100 µg

Anti-Human IL-3 Rα (CD123) - 100 µg

Item no.	LEIN-I-651-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunocytochemistry Primary Antibodies Immunochemistry
Type	Antibody Monoclonal
Applications	WB, FC, IHC, ICC
Clone	32703
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Garnache-Ottou, F. et al. (2015) Haematologica. 100(2):223-30 2. Phillips, GL. et al. (2000) Leukemia 14: 1777
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, FC, ICC, IHC FFPE, N, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	IL-3 R alpha
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a type I transmembrane protein that is expressed on the large majority of progenitor cells. IL-3R alpha function can be defined by the activity of its ligand interleukin-3. IL-3 is a multipotent cytokine that promotes the development of hemopoietic progenitors into cells of the erythroid, myeloid and lymphoid lineages.1 CD123 may be a promising strategy for the preferential ablation of acute myelogenous leukemia (AML) cells.2
Background	Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a type I transmembrane protein that is expressed on the large majority of progenitor cells. IL-3R alpha function can be defined by the activity of its ligand interleukin-3. IL-3 is a multipotent cytokine that promotes the development of hemopoietic progenitors into cells of the erythroid, myeloid and lymphoid lineages.¹ CD123 may be a promising strategy for the preferential ablation of acute myelogenous leukemia (AML) cells.²
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human IL-3 Rα bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-3 Rα (at 0.5 ng/ml) is 0.6 - 1.2 µg/ml. CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. mmunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
PubMed	IL-3 R alpha
Manufacturer - Specificity	Mouse Anti-Human Interleukin 3 Soluble Receptor Alpha (IL-3 Rα) (Clone 32703) recognizes an epitope on Human IL-3 Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2830773
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human IL-3 Rα (Accession # P26951)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Interleukin-3 Soluble Receptor Alpha, IL3RA, CD123, IL3R, IL3RAY, IL3RX, IL3RY, MGC34174, hIL-3Ra, 32703, IL-3 Rα, Human, Anti-Human IL-3 Rα, CD123 antigen, CD123, hIL3Ra, hIL-3Ra, IL-3 R alpha, IL-3 receptor subunit alpha, IL3R alpha, IL-3R subunit alpha, IL3R, IL3RA, IL-3Ra, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, interleukin 3 receptor, alpha (low affinity), interleukin-3 receptor subunit alpha, MGC34174

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-651-100ug-safety-datasheet.pdf