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Anti-Human ICAM-3 (Clone 76205) - 25 µg

Anti-Human ICAM-3 (Clone 76205) - 25 µg

Item no.	LEIN-I-713-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	25 ug 500 ug
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, other
Clone	76205
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Simmons, DL. et al. (1992) Nature 360: 481 2. Mason, DY. et al. (1994) J Clin Pathol 47: 143
NCBI	3385
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	B, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	ICAM-3
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50) is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins that bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule. ICAM-3 is potentially the most important ligand for LFA-1 in the initiation of the immune response because the expression of ICAM-1 on resting leukocytes is low.1 ICAM-3 is closely related to ICAM-1. 1 ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.2
Background	Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50) is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins that bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule. ICAM-3 is potentially the most important ligand for LFA-1 in the initiation of the immune response because the expression of ICAM-1 on resting leukocytes is low.¹ ICAM-3 is closely related to ICAM-1. ¹ ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.²
Other Applications Reported In Literature	Blocking:The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
PubMed	ICAM-3
Manufacturer - Specificity	Mouse Anti-Human Intercellular Adhesion Molecule 3 (ICAM-3) (Clone 76205) recognizes an epitope on Human ICAM-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhICAM-1 or rhICAM-2.
RRID	AB_2830834
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	NS0-Derived Recombinant Human ICAM Extracellular Domain (Accession # CAA49473)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Intercellular Adhesion Molecule 3, CD50, CDW50, ICAM-R, 76205, ICAM-3, Human, Anti-Human ICAM-3, CD50 antigen, CD50, CDW50CDw50, ICAM3, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-713-25ug-safety-datasheet.pdf